Work with us

The Stem Cell Hotel is configured to support and enable scientists from King's and other universities, and researchers working across regenerative medicine.  It incubates the 'next generation' technologies that deliver breakthrough benefit to the industry.

The Stem Cell Hotel is a key interface between King's and the cell therapy, regenerative medicine, drug discovery and phenotyping communities. It is at the forefront of the move to 'data-driven research' complementing 'hypothesis-driven research.'

It is a source of expertise, from which researchers in academia and industry can seek assistance in resolving specific technical problems.  To talk to us about how we can support you, or to enquire about time on one of our devices, please CLICK HERE

Resources and expertise around stem cell biology, high content imaging and artificial micro-environments for cell culture are provided to users in academia and biotechnology industry as a service. This ranges from assistance for assay development and image acquisition to dedicated data analysis and integration.

The operation also leverages innovative research projects currently ongoing at the Centre such as the development of standard methods for characterisation of induced Pluripotent Stem Cells (iPSCs). Technologies from several providers include methods for microscopy imaging, cell-based assays, materials, high content analysis and data integration.

Born within the framework of the Human Induced Pluripotent Stem Cell Initiative (HIPSCI), this collaborative phenotyping space intends to foster and serve communities centred around academic, clinical and commercial research. Below you can find a list of recent publications that benefit from the use of our capabilities to acquire and analyse imaging data.

    Selected Publications

    Geraldine M. Jowett, Michael D. A. Norman, Tracy T. L. Yu, Patricia Rosell Arévalo, Dominique Hoogland, Suzette Lust, Emily Read, Eva Hamrud, Nick J. Walters, Umar Niazi, Matthew Wai Heng Chung, Daniele Marciano, Omer Serhan Omer, Tomasz Zabinski, Davide Danovi, Graham M. Lord, Jöns Hilborn, Nicholas D. Evans, Cécile A. Dreiss, Laurent Bozec, Oommen P. Oommen, Christian D. Lorenz, Ricardo M.P. da Silva, Joana F. Neves, Eileen Gentleman 'ILC1-derived TGFβ1 drives intestinal remodellingNat Mater. 2020 Sep 7. doi: 10.1038/s41563-020-0783-8

    Haneen Alsehli, Fuad Mosis, Christopher Thomson, Eva Hamrud, Erika Wiseman, Eileen Gentleman, Davide Danovi 'An integrated pipeline for high-throughput screening and profiling of spheroids using simple live image analysis of frame to frame variations' Methods 2020-05 | DOI: 10.1016/j.ymeth.2020.05.017

    Wiseman E, Zamuner A, Tang Z, Rogers J, Munir S, Di Silvio L, Danovi D, Veschini L. 'Integrated Multiparametric High-Content Profiling of Endothelial Cells.' SLAS Discov. 2019 Mar;24(3):264-273. doi: 10.1177/2472555218820848. Epub 2019 Jan 25.

    Vigilante, A., Laddach, A., Moens, N., Meleckyte, R., Leha, A., Ghahramani, A., Culley, O. J., Kathuria, A., Hurling, C., Vickers, A., Wiseman, E., Tewary, M., Zandstra, P. W., Durbin, R., Fraternali, F., Stegle, O., Birney, E., Luscombe, N. M., HipSci Consortium, Danovi, D. & Watt F. Identifying Extrinsic versus Intrinsic Drivers of Variation in Cell Behavior in Human iPSC Lines from Healthy Donors 19 Feb 2019, In : Cell Reports. 26, 8, p. 2078-2087.e3

    Ong J, Serra MP, Segal J, Cujba AM, Ng SS, Butler R, Millar V, Hatch S, Zimri S, Koike H, Chan K, Bonham A, Walk M, Voss T, Heaton N, Mitry R, Dhawan A, Ebner D, Danovi D, Nakauchi H, Rashid ST.  'Imaging-Based Screen Identifies Laminin 411 as a Physiologically Relevant Niche Factor with Importance for i-Hep Applications.' Stem Cell Reports. 2018 Mar 13;10(3):693-702. doi: 10.1016/j.stemcr.2018.01.025. Epub 2018 Mar 1.

    Ghahramani A, Donati G, Luscombe NM, Watt FM. 'Epidermal Wnt signalling regulates transcriptome heterogeneity and proliferative fate in neighbouring cells.' Genome Biol. 2018 Jan 15;19(1):3. doi: 10.1186/s13059-017-1384-y. PMID: 29334988

    Kilpinen H, Goncalves A, Leha A, Afzal V, Alasoo K, Ashford S, Bala S, Bensaddek D, Casale FP, Culley OJ, Danecek P, Faulconbridge A, Harrison PW, Kathuria A, McCarthy D, McCarthy SA, Meleckyte R, Memari Y, Moens N, Soares F, Mann A, Streeter I, Agu CA, Alderton A, Nelson R, Harper S, Patel M, White A, Patel SR, Clarke L, Halai R, Kirton CM, Kolb-Kokocinski A, Beales P, Birney E, Danovi D, Lamond AI, Ouwehand WH, Vallier L, Watt FM, Durbin R, Stegle O, Gaffney DJ. 'Corrigendum: Common genetic variation drives molecular heterogeneity in human iPSCs.' Nature. 2017 Jun 29;546(7660):686. doi: 10.1038/nature23012. PMID: 28614302

    Walko G, Woodhouse S, Pisco AO, Rognoni E, Liakath-Ali K, Lichtenberger BM, Mishra A, Telerman SB, Viswanathan P, Logtenberg M, Renz LM, Donati G, Quist SR, Watt FM. 'A genome-wide screen identifies YAP/WBP2 interplay conferring growth advantage on human epidermal stem cells.' Nat Commun. 2017 Mar 23;8:14744. doi: 10.1038/ncomms14744. PMID: 28332498

    Hayes TF, Benaich N, Goldie SJ, Sipilä K, Ames-Draycott A, Cai W, Yin G, Watt FM. 'Integrative genomic and functional analysis of human oral squamous cell carcinoma cell lines reveals synergistic effects of FAT1 and CASP8 inactivation.' Cancer Lett. 2016 Dec 1;383(1):106-114. doi: 10.1016/j.canlet.2016.09.014. PMID: 27693639

    Kerz M, Folarin A, Meleckyte R, Watt FM, Dobson RJ, Danovi D. 'A Novel Automated High-Content Analysis Workflow Capturing Cell Population Dynamics from Induced Pluripotent Stem Cell Live Imaging Data.' J Biomol Screen. 2016 Jun 2. pii: 1087057116652064. PMID: 27256155

    Reimer A, Vasilevich A, Hulshof F, Viswanathan P, van Blitterswijk CA, de Boer J, Watt FM. 'Scalable topographies to support proliferation and Oct4 expression by human induced pluripotent stem cells.' Sci Rep. 2016 Jan 13;6:18948. doi: 10.1038/srep18948. PMID: 26757610

    Viswanathan P, Guvendiren M, Chua W, Telerman SB, Liakath-Ali K, Burdick JA, Watt FM. 'Mimicking the topography of the epidermal-dermal interface with elastomer substrates.' Integr Biol (Camb). 2015 Dec 11. Viswanathan P, Guvendiren M, Chua W, Telerman SB, Liakath-Ali K, Burdick JA, Watt FM. PMID: 26658424

    Leha A, Moens N, Meleckyte R, Culley OJ, Gervasio MK, Kerz M, Reimer A, Cain SA, Streeter I, Folarin A, Stegle O, Kielty CM; HipSci Consortium, Durbin R, Watt FM, Danovi D. 'A high-content platform to characterise human induced pluripotent stem cell lines.' Methods. 2015 Nov 25. pii: S1046-2023(15)30153-5. doi: 10.1016/j.ymeth.2015.11.012. PMID: 26608109