PerkinElmer Operetta CLS™ High-Content Analysis System allows users to reveal fine sub-cellular details at high speed, sensitivity, resolution, and flexibility.Hardware
Transmitted light capabilities (brightfield, digital, phase contrast).
Widefield and confocal fluorescence.
The instrument comes with the Harmony® 4.8 High-Content Imaging and Analysis Software for the set-up of assays, automation of experiments. image acquisition, data analysis, and storage and retrieval of results (all in a simple, powerful and easy way). The software allows:
- Computer controlled excitation power
- High-speed autofocus in every field.
- Full exposure control and channel editor.
- Simultaneous acquisition and analysis, and visualization of data during acquisition.
- Variable plate formats: 6, 24, 48, 96, 384, 1536-well.
- Multi-field imaging acquisition.
- Z-sectioning for identification of the z-plane of interest.
- Automated flat field correction.
- Time kinetics capabilities.
- Fully automated cell segmentation with automatic or manual parameter tuning.
- Ready-made turnkey applications.
- Easy and intuitive creation and modification of analysis sequences.
- PhenoLOGICTM machine learning plug-in to teach the software to create tailored algorithms.
- Texture analysis capabilities.
- Scalable database and search via sortable tree.
- Automatic storage of metadata and additional experiment information.
- Allows for image storage on a remote server.
- Export results automatically or in batches into ColumbusTM image data storage and analysis system. (allows access, re-analysis, storage, and share of data).
- Export results for further analysis (i.e.: High Content ProfilerTM platform by TIBCO Spotfire).
- Accessible from office installations of the Harmony software for data inspection, analysis, and result export.
Regarding the applications of the instrument, examples are:
- Fixed-cell assays.
- Live-cell assays.
- Imaging of complex cellular models.
- Advanced assays such as FRET.
- Phenotypic fingerprinting.
Image courtesy of Marian Seda, Ph.D