Operetta CLS

PerkinElmer Operetta CLS™ High-Content Analysis System allows users to reveal fine sub-cellular details at high speed, sensitivity, resolution, and flexibility.
Hardware
- Transmitted light capabilities (brightfield,
digital, phase contrast).
Widefield and confocal fluorescence. - Powerful, stable 8x LED light source for optimal excitation of fluorophores and confidence in results.
- Automated water-immersion objectives with a very high numerical aperture for high resolution and fast read times with minimal photodamage.
- Confocal spinning disk technology which allows a fast imaging process, efficient background rejection, live-cell experiments and 3D imaging.
- Large format 16-bit sCMOS camera (4.7 Megapixel, 2160x2160, 6.5 microns pixel size) which ensures low noise, wide dynamic range and high resolution (50nm) for sensitive and quantitative measurements at short exposure times.
- User-accessible bar-coded emission filters provide flexibility, sensitive imaging, and error-free operation.
Software
Harmony® 4.9 High-Content Imaging and Analysis Software for the set-up of assays, automation of experiments, image acquisition, data analysis, and storage and retrieval of results.
Image acquisition
- Computer-controlled excitation power
- High-speed autofocus in every field.
- Full exposure control and channel editor.
- Simultaneous acquisition and analysis, and visualization of data during acquisition.
- Variable formats: slides, multi-well plates from 6 wells up to 1536.
- Multi-field imaging acquisition.
- Z-sectioning for identification of the z-plane of interest.
- Automated flat-field correction.
- Time kinetics capabilities.
- PreciScanTM Intelligent image acquisition: Target more accurately your object of interest for significantly reduced acquisition and analysis times, particularly valuable for 3D microtissue and rare event studies.
Image analysis
- Fully automated cell segmentation with automatic or manual parameter tuning.
- Ready-made turnkey applications.
- Easy and intuitive creation and modification of analysis sequences.
- PhenoLOGICTM machine learning plug-in to teach the software to create tailored algorithms.
- Texture analysis capabilities.
Data management
- Scalable database and search via sortable tree.
- Automatic storage of metadata and additional experiment information.
- Allows for image storage on a remote server.
- Export results automatically or in batches into ColumbusTM image data storage and analysis system. (allows access, re-analysis, storage, and sharing of data).
- Export results for further analysis such as High Content ProfilerTM platform by TIBCO Spotfire.
- Accessible from office installations of the Harmony software for data inspection, analysis, and result export.
Applications
Regarding the applications of the instrument, examples are:
- Live-cell and fixed-cell assays.
- Imaging of complex cellular models.
- 3D imaging (organoids, spheroids, tissue sections).
- Advanced assays such as FRET.
- Cell Painting and phenotypic fingerprinting.
Images courtesy of M.Cristina Garrone, Theoni Demcollari, Thomas Williams