Operetta CLS

PerkinElmer Operetta CLS™ High-Content Analysis System allows users to reveal fine sub-cellular details at high speed, sensitivity, resolution, and flexibility.

Hardware

  • Transmitted light capabilities (brightfield, digital, phase contrast).
    Widefield and confocal fluorescence.
  • Powerful, stable 8x LED light source for optimal excitation of fluorophores and confidence in results.
  • Automated water-immersion objectives with a very high numerical aperture for high resolution and fast read times with minimal photodamage.
  • Confocal spinning disk technology which allows a fast imaging process, efficient background rejection, live-cell experiments and 3D imaging.
  • Large format 16-bit sCMOS camera (4.7 Megapixel, 2160x2160, 6.5 microns pixel size) which ensures low noise, wide dynamic range and high resolution (50nm) for sensitive and quantitative measurements at short exposure times.
  • User-accessible bar-coded emission filters provide flexibility, sensitive imaging, and error-free operation.

Software

Harmony® 4.9 High-Content Imaging and Analysis Software for the set-up of assays, automation of experiments, image acquisition, data analysis, and storage and retrieval of results.

Image acquisition

  • Computer-controlled excitation power
  • High-speed autofocus in every field.
  • Full exposure control and channel editor.
  • Simultaneous acquisition and analysis, and visualization of data during acquisition.
  • Variable formats: slides, multi-well plates from 6 wells up to 1536.
  • Multi-field imaging acquisition.
  • Z-sectioning for identification of the z-plane of interest.
  • Automated flat-field correction.
  • Time kinetics capabilities.
  • PreciScanTM Intelligent image acquisition: Target more accurately your object of interest for significantly reduced acquisition and analysis times, particularly valuable for 3D microtissue and rare event studies.

Image analysis

  • Fully automated cell segmentation with automatic or manual parameter tuning.
  • Ready-made turnkey applications.
  • Easy and intuitive creation and modification of analysis sequences.
  • PhenoLOGICTM machine learning plug-in to teach the software to create tailored algorithms.
  • Texture analysis capabilities.

Data management

  • Scalable database and search via sortable tree.
  • Automatic storage of metadata and additional experiment information.
  • Allows for image storage on a remote server.
  • Export results automatically or in batches into ColumbusTM image data storage and analysis system. (allows access, re-analysis, storage, and sharing of data).
  • Export results for further analysis such as High Content ProfilerTM platform by TIBCO Spotfire.
  • Accessible from office installations of the Harmony software for data inspection, analysis, and result export.

Applications

Regarding the applications of the instrument, examples are:

  • Live-cell and fixed-cell assays.
  • Imaging of complex cellular models.
  • 3D imaging (organoids, spheroids, tissue sections).
  • Advanced assays such as FRET.
  • Cell Painting and phenotypic fingerprinting.

Images courtesy of M.Cristina Garrone, Theoni Demcollari, Thomas Williams