Work with us
The Stem Cell Hotel is configured to support and enable
scientists from King's and other universities, and researchers working across
regenerative medicine. It incubates and launches the 'next generation' technologies
that, once adopted, deliver breakthrough benefit to the industry.
The Stem Cell Hotel is a key interface between King's and the cell therapy, regenerative medicine, drug discovery and phenotyping communities. It is at the forefront of the move to 'data-driven research' that compliments traditional 'hypothesis-driven research.'
It is a source of expertise, from which researchers in academia and industry can seek assistance in resolving specific technical problems. To talk to us about how we can support you, or to enquire about accessing time on one of our devices, please CLICK HERE
Resources and expertise around stem cell biology, artificial microenvironments for cell culture and high content imaging are provided to users as a service. This includes assistance for assay development, image acquisition and dedicated data analysis and integration.
The operation also leverages innovative research projects currently ongoing at the Centre such as the development of standard methods for characterisation of induced Pluripotent Stem Cells (iPSCs). Technologies from several providers include methods for microscopy imaging, cell-based assays, materials, high content analysis and data integration.
Born within the framework of the Human Induced Pluripotent Stem Cell Initiative (HIPSCI), this collaborative phenotyping space intends to foster and serve communities centred around academic, clinical and commercial research. Below you can find a list of recent publications that benefit from the use of our capabilities to acquire and analyse imaging data.
HUMAN INDUCED PLURIPOTENT STEM CELL INITIATIVE
Wiseman E, Zamuner A, Tang Z, Rogers J, Munir S, Di Silvio L, Danovi D, Veschini L. 'Integrated Multiparametric High-Content Profiling of Endothelial Cells.' SLAS Discov. 2019 Mar;24(3):264-273. doi: 10.1177/2472555218820848. Epub 2019 Jan 25.
Ghahramani A, Donati G, Luscombe NM, Watt FM. 'Epidermal Wnt signalling regulates transcriptome heterogeneity and proliferative fate in neighbouring cells.' Genome Biol. 2018 Jan 15;19(1):3. doi: 10.1186/s13059-017-1384-y. PMID: 29334988
Kilpinen H, Goncalves A, Leha A, Afzal V, Alasoo K, Ashford S, Bala S, Bensaddek D, Casale FP, Culley OJ, Danecek P, Faulconbridge A, Harrison PW, Kathuria A, McCarthy D, McCarthy SA, Meleckyte R, Memari Y, Moens N, Soares F, Mann A, Streeter I, Agu CA, Alderton A, Nelson R, Harper S, Patel M, White A, Patel SR, Clarke L, Halai R, Kirton CM, Kolb-Kokocinski A, Beales P, Birney E, Danovi D, Lamond AI, Ouwehand WH, Vallier L, Watt FM, Durbin R, Stegle O, Gaffney DJ. 'Corrigendum: Common genetic variation drives molecular heterogeneity in human iPSCs.' Nature. 2017 Jun 29;546(7660):686. doi: 10.1038/nature23012. PMID: 28614302
Walko G, Woodhouse S, Pisco AO, Rognoni E, Liakath-Ali K, Lichtenberger BM, Mishra A, Telerman SB, Viswanathan P, Logtenberg M, Renz LM, Donati G, Quist SR, Watt FM. 'A genome-wide screen identifies YAP/WBP2 interplay conferring growth advantage on human epidermal stem cells.' Nat Commun. 2017 Mar 23;8:14744. doi: 10.1038/ncomms14744. PMID: 28332498
Hayes TF, Benaich N, Goldie SJ, Sipilä K, Ames-Draycott A, Cai W, Yin G, Watt FM. 'Integrative genomic and functional analysis of human oral squamous cell carcinoma cell lines reveals synergistic effects of FAT1 and CASP8 inactivation.' Cancer Lett. 2016 Dec 1;383(1):106-114. doi: 10.1016/j.canlet.2016.09.014. PMID: 27693639
Kerz M, Folarin A, Meleckyte R, Watt FM, Dobson RJ, Danovi D. 'A Novel Automated High-Content Analysis Workflow Capturing Cell Population Dynamics from Induced Pluripotent Stem Cell Live Imaging Data.' J Biomol Screen. 2016 Jun 2. pii: 1087057116652064. PMID: 27256155
Reimer A, Vasilevich A, Hulshof F, Viswanathan P, van Blitterswijk CA, de Boer J, Watt FM. 'Scalable topographies to support proliferation and Oct4 expression by human induced pluripotent stem cells.' Sci Rep. 2016 Jan 13;6:18948. doi: 10.1038/srep18948. PMID: 26757610
Viswanathan P, Guvendiren M, Chua W, Telerman SB, Liakath-Ali K, Burdick JA, Watt FM. 'Mimicking the topography of the epidermal-dermal interface with elastomer substrates.' Integr Biol (Camb). 2015 Dec 11. Viswanathan P, Guvendiren M, Chua W, Telerman SB, Liakath-Ali K, Burdick JA, Watt FM. PMID: 26658424
Leha A, Moens N, Meleckyte R, Culley OJ, Gervasio MK, Kerz M, Reimer A, Cain SA, Streeter I, Folarin A, Stegle O, Kielty CM; HipSci Consortium, Durbin R, Watt FM, Danovi D. 'A high-content platform to characterise human induced pluripotent stem cell lines.' Methods. 2015 Nov 25. pii: S1046-2023(15)30153-5. doi: 10.1016/j.ymeth.2015.11.012. PMID: 26608109
Stem Cell Reports. 2018 Mar 13;10(3):693-702. doi: 10.1016/j.stemcr.2018.01.025. Epub 2018 Mar 1. 'Imaging-Based Screen Identifies Laminin 411 as a Physiologically Relevant Niche Factor with Importance for i-Hep Applications.' Ong J1, Serra MP1, Segal J1, Cujba AM1, Ng SS1, Butler R2, Millar V3, Hatch S3, Zimri S1, Koike H4, Chan K4, Bonham A4, Walk M5, Voss T5, Heaton N1, Mitry R1, Dhawan A1, Ebner D3, Danovi D1, Nakauchi H2, Rashid ST6.
Image courtesy Jennifer Coleman, Ph.D Student